Różnica między sumą mas nukleonów, wchodzących w skład jądra atomowego, a masą jądra. Iloczyn defektu masy i kwadratu prędkości światła w próżni jest równy energii wiązania jądra (ze wzoru Einsteina: E = Δm‧c2, E – energia wiązania jądra, Δm – defekt masy, c – prędkość światła w próżni).
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